Metal-organic frameworks (MOFs) are the crystalline compounds obtained by complexing various metal ions and ligands. The MOFs display unique properties, such as ultrahigh porosity and bulk volume. These materials can be prepared by a solvothermal wet-chemistry method to achieve well-defined single crystals with tunable shape and size. The organic linkers (also called ligands) allow for formation of diversified pore size, topology and surface area (> 6000 g/m2 as reported). The ligands used to produce MOFs in this study are amphiprotic, including carboxylic and amino functional groups. In this thesis, the MOFs derived from the solvothermal chemistry have been extensively studied using two standard cytotoxicity measurements. The nitrogen oxide (NO), which involves in neuronal transmission and modulation in central nervous systems (CNS), was monitored to provide understanding on the cytotoxicity of MOF motifs towards the retinal pigment epithelial (RPE) cells. The lactate dehydrogenase (LDH) assay using the RPE whole cells was also carried out to determine whether or not the biochemical pathways are dramatically altered. Additionally, the mitochondrial membrane potential (MMP), reactive oxygen species (ROS), and singlet oxygen species (SOS) were measured to determine cell viability and observe cytotoxicity of these MOFs. The cytotoxicity using commercially available anti-cancer drug, 7-Ethyl-10-hydroxy-camptothecin (C22H20N2O5, SN-38) was also evaluated for comparison to understand the inhibition of DNA synthesis and damage to the plasma membrane.
January 30, 2015
The right to download or print any of the pages of this thesis (Material) is granted by the copyright owner only for personal or classroom use. The author retains all proprietary rights, including copyright ownership. Any reproduction or editing or other use of this Material by any means requires the express written permission of the copyright owner. Except as provided above, or any use beyond what is allowed by fair use (Title 17 Section 107 U.S.C.), you may not reproduce, republish, post, transmit or distribute any Material from this web site in any physical or digital form without the permission of the copyright owner of the Material. Inquiries regarding any further use of these materials should be addressed to Administration, Jernigan Library, Texas A&M University-Kingsville, 700 University Blvd. Kingsville, Texas 78363-8202, (361)593-3416.